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Last updated: 2016/12/11
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SPANXB1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00728695-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-SPANXB1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 11.8
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-SPANXB1 antibody (H00728695-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00728695-T01
    transfected lysate.
    Western Blot
    QC Testing of H00728695-T01
    Lane 1: transfected lysate ( 11.80 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Gene Name:
  • SPANXB1
  • Gene Alias:
  • B1,SPANX-B,SPANXB
  • Gene Description:
  • SPANX family, member B1
  • Gene Summary:
  • Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-tail orientation with SPANX family member B2, which appears to be a duplication of the B1 locus. The SPANXB genes are unique members of this gene family, since they contain an additional 18 nt in their coding region compared to the majority of family members. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000024171,nuclear-associated protein SPAN-Xb,sperm protein associated with the nucleus, X chromosome, family member B1
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