NANOGP8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NANOGP8 transfected lysate.
Western Blot
Lane 1: NANOGP8 transfected lysate ( 34.60 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NANOGP8 full-length
Host
Human
Theoretical MW (kDa)
34.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NANOGP8 antibody (H00388112-B01P) by Western Blots.
SDS-PAGE Gel
NANOGP8 transfected lysate.
Western Blot
Lane 1: NANOGP8 transfected lysate ( 34.60 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NANOGP8
Entrez GeneID
388112GeneBank Accession#
BC069807Protein Accession#
AAH69807Gene Name
NANOGP8
Gene Alias
MGC119250, NANOG, NANOGP1
Gene Description
Nanog homeobox pseudogene 8
Gene Ontology
HyperlinkGene Summary
This locus is a processed pseudogene of the transcription factor NANOG. NANOG plays a central role in regulating self-renewal in pluripotent stem cells and tumor cells. This pseudogene contains an intact open reading frame that could potentially encode a protein similar to NANOG. Although there is no evidence of transcription from this pseudogene, RT-PCR studies suggest that NANOGP8 may be expressed in some cancer cell lines. In vitro studies using a recombinant NANOGP8 protein have shown that the protein localizes to the nucleus and can promote cell proliferation, similar to NANOG. [provided by RefSeq
Other Designations
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