SUMO4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
SUMO4 transfected lysate.
Western Blot
Lane 1: SUMO4 transfected lysate ( 10.56 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-SUMO4 full-length
Host
Human
Theoretical MW (kDa)
10.56
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-SUMO4 antibody (H00387082-B01) by Western Blots.
SDS-PAGE Gel
SUMO4 transfected lysate.
Western Blot
Lane 1: SUMO4 transfected lysate ( 10.56 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — SUMO4
Entrez GeneID
387082GeneBank Accession#
NM_001002255.1Protein Accession#
NP_001002255.1Gene Name
SUMO4
Gene Alias
IDDM5, SMT3H4, SUMO-4, dJ281H8.4
Gene Description
SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization, stability, or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. A specific polymorphism in this SUMO gene, which leads to the M55V substitution, has been associated with type I diabetes. The RefSeq contains this polymorphism. [provided by RefSeq
Other Designations
OTTHUMP00000017390|SMT3 suppressor of mif two 3 homolog 2|SMT3 suppressor of mif two 3 homolog 4|small ubiquitin-like modifier 4 protein|small ubiquitin-like protein 4
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Disease
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