DUB3 monoclonal antibody (M01), clone 3G12
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant DUB3.
Immunogen
DUB3 (NP_958804, 431 a.a. ~ 530 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ESTLDHWKFLQEQNKTKPEFNVRKVEGTLPPDVLVIHQSKYKCGMKNHHPEQQSSLLNLSSTTPTHQESMNTGTLASLRGRARRSKGKNKHSKRALLVCQ
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DUB3 is approximately 0.3ng/ml as a capture antibody.ELISA
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Gene Info — DUB3
Entrez GeneID
377630GeneBank Accession#
NM_201402Protein Accession#
NP_958804Gene Name
DUB3
Gene Alias
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Gene Description
deubiquitinating enzyme 3
Omim ID
610186Gene Ontology
HyperlinkGene Summary
DUB3 is a member of the ubiquitin processing protease (UBP) subfamily of deubiquitinating enzymes. See USP1 (MIM 603478) for background information.[supplied by OMIM
Other Designations
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Interactome
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Publication Reference
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BRE/BRCC45 regulates CDC25A stability by recruiting USP7 in response to DNA damage.
Biswas K, Philip S, Yadav A, Martin BK, Burkett S, Singh V, Babbar A, North SL, Chang S, Sharan SK.
Nature Communications 2018 Feb; 9(1):537.
Application:WB-Tr, Human, MCF-7 cells.
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BRE/BRCC45 regulates CDC25A stability by recruiting USP7 in response to DNA damage.
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