NEK8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NEK8 transfected lysate.
Western Blot
Lane 1: NEK8 transfected lysate ( 76.23 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NEK8 full-length
Host
Human
Theoretical MW (kDa)
76.23
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NEK8 antibody (H00284086-B01) by Western Blots.
SDS-PAGE Gel
NEK8 transfected lysate.
Western Blot
Lane 1: NEK8 transfected lysate ( 76.23 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NEK8
Entrez GeneID
284086GeneBank Accession#
NM_178170.2Protein Accession#
NP_835464.1Gene Name
NEK8
Gene Alias
JCK, MGC138445, NEK12A
Gene Description
NIMA (never in mitosis gene a)- related kinase 8
Omim ID
609799Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans. [provided by RefSeq
Other Designations
NIMA-family kinase NEK8|NIMA-related kinase 12a|NIMA-related kinase 8|serine/thrionine-protein kinase NEK8
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