P4HA3 monoclonal antibody (M02), clone 7F10
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant P4HA3.
Immunogen
P4HA3 (NP_878907.1, 66 a.a. ~ 165 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
TRFYDKVLSLHEDSTTPVANPLLAFTLIKRLQSDWRNVVHSLEASENIRALKDGYEKVEQDLPAFEDLEGAARALMRLQDVYMLNVKGLARGVFQRVTGS
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged P4HA3 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — P4HA3
Entrez GeneID
283208GeneBank Accession#
NM_182904Protein Accession#
NP_878907.1Gene Name
P4HA3
Gene Alias
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Gene Description
prolyl 4-hydroxylase, alpha polypeptide III
Omim ID
608987Gene Ontology
HyperlinkGene Summary
This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. [provided by RefSeq
Other Designations
C-P4H alpha III|collagen prolyl 4-hydroxylase alpha(III)|procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide III|prolyl 4-hydroxylase, alpha III subunit
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Interactome
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Pathway
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Disease
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