NCR3 (Human) Recombinant Protein
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Specification
Product Description
Purified NCR3 (NP_667341.1 19 a.a. - 135 a.a.) human recombinant protein with His-Flag-StrepII tag at N-terminus expressed in human cells.
Transfected Cell Line
Human HEK293H cells
Sequence
LWVSQPPEIRTLEGSSAFLPCSFNASQGRLAIGSVTWFRDEVVPGKEVRNGTPEFRGRLAPLASSRFLHDHQAELHIRDVRGHDASIYVCRVEVLGLGVGTGNGTRLVVEKEHPQLG
Host
Human
Theoretical MW (kDa)
18.15
Form
Liquid
Preparation Method
Transfection of pSuper-NCR3 plasmid into HEK293H cell, and the expressed protein was purified by Strep-Tactin affinity column.
Purification
Strep-Tactin affinity columns
Concentration
≥ 10 ug/ml
Storage Buffer
100 mM Tris-HCl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 5 mM desthiobiotin.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Enzyme-linked Immunoabsorbent Assay
SDS-PAGE
Protein Interaction
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Gene Info — NCR3
Entrez GeneID
259197GeneBank Accession#
NM_147130.1Protein Accession#
NP_667341.1Gene Name
NCR3
Gene Alias
1C7, CD337, LY117, MALS, NKp30
Gene Description
natural cytotoxicity triggering receptor 3
Omim ID
611550Gene Ontology
HyperlinkGene Summary
Natural cytotoxicity receptors (NCRs), such as NCR3, are activating natural killer (NK) cell receptors that belong to the immunoglobulin (Ig) superfamily. NCR3 is expressed in all resting and activated NK cells and forms a complex with CD3-zeta (CD3Z, or CD247; MIM 186780) (Sato et al., 2001 [PubMed 11782277]).[supplied by OMIM
Other Designations
OTTHUMP00000029162|OTTHUMP00000038859|OTTHUMP00000038863|lymphocyte antigen 117
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