ANKS4B monoclonal antibody (M03), clone 3E3
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More Files
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Western Blot (Tissue lysate)
ANKS4B monoclonal antibody (M03), clone 3E3. Western Blot analysis of ANKS4B expression in human kidney.
Western Blot (Cell lysate)
ANKS4B monoclonal antibody (M03), clone 3E3. Western Blot analysis of ANKS4B expression in K-562.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ANKS4B is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (73 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant ANKS4B.
Immunogen
ANKS4B (NP_665872.1, 1 a.a. ~ 417 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSTRYHQAASDSYLELLKEATKRDLNLSDEDGMTPTLLAAYHGNLEALEIICSRGGDPDRCDIWGNTPLHFAASNGHAHCVSFLVNFGANIFALDNDLQTPLDAAASREQNECVALLDKAATAQNIMNPKKVTRLKEQAQKNARRQIKECERLQEKHQNKMAHTYSKEESGTLSSSKGTFSRSSPSNASAPGTFGSLSKGIKDTFKIKFKKNKDTAEQVGKEGRSGQRNVMEVFREEEEDSFSGDFKEKLQLSAEEDGSVHHESILNRPGLGSIVFRRNRISSPEDISDSKREFGFKLPSELLQRQGASEADEGAADEEGEENGLKDDLPWDDDEVEWEEDVVDATPLEVFLLSQHLEEFLPIFKREQIDLEALLLCSDEDLQSIQMQLGPRKKVLNAINRRKQVLQQPGQLVDTSL
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
ANKS4B monoclonal antibody (M03), clone 3E3. Western Blot analysis of ANKS4B expression in human kidney.Western Blot (Cell lysate)
ANKS4B monoclonal antibody (M03), clone 3E3. Western Blot analysis of ANKS4B expression in K-562.Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ANKS4B is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — ANKS4B
Entrez GeneID
257629GeneBank Accession#
NM_145865.1Protein Accession#
NP_665872.1Gene Name
ANKS4B
Gene Alias
FLJ38819, HARP, MGC133380, MGC133381
Gene Description
ankyrin repeat and sterile alpha motif domain containing 4B
Omim ID
609901Gene Ontology
HyperlinkOther Designations
harmonin-interacting ankyrin-repeat containing protein
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Publication Reference
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A cryptic sequence targets the adhesion complex scaffold ANKS4B to apical microvilli to promote enterocyte brush border assembly.
Maura J Graves, Samaneh Matoo, Myoung Soo Choi, Zachary A Storad, Rawnag A El Sheikh Idris, Brooke K Pickles, Prashun Acharya, Paula E Shinder, Taylen O Arvay, Scott William Crawley.
The Journal of Biological Chemistry 2020 Sep; 295(36):12588.
Application：IF, IHC-P, WB-Tr, Human, Mouse, Caco-2 cells, HEK 293T cells, Mouse intestinal tissues.
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A cryptic sequence targets the adhesion complex scaffold ANKS4B to apical microvilli to promote enterocyte brush border assembly.
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