NEIL2 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NEIL2 full-length
Host
Human
Theoretical MW (kDa)
36.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NEIL2 antibody (H00252969-D01) by Western Blots.
SDS-PAGE Gel
NEIL2 transfected lysate.
Western Blot
Lane 1: NEIL2 transfected lysate ( 36.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NEIL2
Entrez GeneID
252969GeneBank Accession#
NM_145043.1Protein Accession#
NP_659480.1Gene Name
NEIL2
Gene Alias
FLJ31644, MGC2832, MGC4505, NEH2, NEI2
Gene Description
nei like 2 (E. coli)
Omim ID
608933Gene Ontology
HyperlinkGene Summary
NEIL2 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226])[supplied by OMIM
Other Designations
OTTHUMP00000116006|nei-like 2
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Interactome
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Pathway
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Disease
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