IL27 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-IL27 full-length
Host
Human
Theoretical MW (kDa)
27.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-IL27 antibody (H00246778-B01) by Western Blots.
SDS-PAGE Gel
IL27 transfected lysate
Western Blot
Lane 1: IL27 transfected lysate ( 27.5 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — IL27
Entrez GeneID
246778GeneBank Accession#
BC062422Protein Accession#
AAH62422Gene Name
IL27
Gene Alias
IL-27, IL-27A, IL27p28, IL30, MGC71873, p28
Gene Description
interleukin 27
Omim ID
608273Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq
Other Designations
IL-27 p28 subunit|OTTHUMP00000122516|interleukin 30|interleukin-27 subunit alpha
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