ATP6V1C2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ATP6V1C2 transfected lysate.
Western Blot
Lane 1: ATP6V1C2 transfected lysate ( 42.02 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ATP6V1C2 full-length
Host
Human
Theoretical MW (kDa)
42.02
Interspecies Antigen Sequence
Mouse (82); Rat (83)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ATP6V1C2 antibody (H00245973-B01) by Western Blots.
SDS-PAGE Gel
ATP6V1C2 transfected lysate.
Western Blot
Lane 1: ATP6V1C2 transfected lysate ( 42.02 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ATP6V1C2
Entrez GeneID
245973GeneBank Accession#
BC012142.1Protein Accession#
AAH12142.1Gene Name
ATP6V1C2
Gene Alias
ATP6C2, VMA5
Gene Description
ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2
Gene Ontology
HyperlinkGene Summary
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A,three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms. [provided by RefSeq
Other Designations
ATPase, H+ transporting, lysosomal 42kD, V1 subunit C|OTTHUMP00000115522|V-ATPase C2 subunit|vacuolar H+ ATPase C2
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Interactome
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Disease
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