ALKBH3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ALKBH3 transfected lysate.
Western Blot
Lane 1: ALKBH3 transfected lysate ( 33.4 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ALKBH3 full-length
Host
Human
Theoretical MW (kDa)
33.4
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ALKBH3 antibody (H00221120-B01) by Western Blots.
SDS-PAGE Gel
ALKBH3 transfected lysate.
Western Blot
Lane 1: ALKBH3 transfected lysate ( 33.4 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ALKBH3
Entrez GeneID
221120GeneBank Accession#
BC103812Protein Accession#
AAI03813Gene Name
ALKBH3
Gene Alias
ABH3, DEPC-1, DEPC1, MGC118790, MGC118792, MGC118793, PCA1
Gene Description
alkB, alkylation repair homolog 3 (E. coli)
Omim ID
610603Gene Ontology
HyperlinkGene Summary
The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM
Other Designations
alkB, alkylation repair homolog 3|prostate cancer antigen-1
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Disease
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