MRPL21 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MRPL21 transfected lysate
Western Blot
Lane 1: MRPL21 transfected lysate ( 23.1 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MRPL21 full-length
Host
Human
Theoretical MW (kDa)
23.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MRPL21 antibody (H00219927-B01) by Western Blots.
SDS-PAGE Gel
MRPL21 transfected lysate
Western Blot
Lane 1: MRPL21 transfected lysate ( 23.1 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MRPL21
Entrez GeneID
219927GeneBank Accession#
BC055088Protein Accession#
AAH55088Gene Name
MRPL21
Gene Alias
L21mt, MGC62013, MRP-L21
Gene Description
mitochondrial ribosomal protein L21
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Multiple transcript variants encoding different isoforms were identified through sequence analysis although some may be subject to nonsense-mediated decay (NMD). [provided by RefSeq
Other Designations
39S ribosomal protein L21, mitochondrial
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