TUBB monoclonal antibody (M04), clone 3B3
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant TUBB.
Immunogen
TUBB (AAH19924, 1 a.a. ~ 444 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGTYHGDSDLQLDRISVYYNEATGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDCLQGFQLTHSLGGGTGSGMGTLLISKIREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSVHQLVENTDETYCIDNEALYDICFRTLKLTTPTYGDLNHLVSATMSGVTTCLRFPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQVFDAKNMMAACDPRHGRYLTVAAVFRGRMSMKEVDEQMLNVQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAVTFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEEDFGEEAEEEA
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (74.36 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
TUBB monoclonal antibody (M04), clone 3B3. Western Blot analysis of TUBB expression in Y-79 ( Cat # L042V1 ).Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to TUBB on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged TUBB is 1 ng/ml as a capture antibody.ELISA
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Gene Info — TUBB
Entrez GeneID
203068GeneBank Accession#
BC019924Protein Accession#
AAH19924Gene Name
TUBB
Gene Alias
M40, MGC117247, MGC16435, OK/SW-cl.56, TUBB1, TUBB5
Gene Description
tubulin, beta
Omim ID
191130Gene Ontology
HyperlinkGene Summary
O
Other Designations
OTTHUMP00000029069|beta 5-tubulin|beta Ib tubulin|beta-4 tubulin|tubulin beta polypeptide|tubulin beta-1 chain|tubulin beta-5 chain|tubulin, beta polypeptide
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Interactome
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Pathway
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Disease
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Publication Reference
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Tubulin acetylation alone does not affect kinesin-1 velocity and run length in vitro.
Walter WJ, Beránek V, Fischermeier E, Diez S.
PLoS One 2012 Aug; 7(8):e42218.
Application:WB-Re, Recombinant protein.
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Immune reactivity to ?]-tubulin isotype 5 and vesicular integral-membrane protein 36 in patients with autoimmune gastrointestinal disorders.
Op De Beeck K, Van den Bergh K, Vermeire S, Decock S, Derua R, Waelkens E, Rutgeerts P, Nevens F, Bossuyt X.
Gut 2011 Nov; 60(11):1601.
Application:ELISA, Human, Serum.
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Tubulin acetylation alone does not affect kinesin-1 velocity and run length in vitro.
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