CENPV monoclonal antibody (M05), clone 1D9
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CENPV.
Immunogen
CENPV (NP_859067.2, 173 a.a. ~ 272 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
ICKKKQNRHFIVPASRFKLLKGAEHITTYTFNTHKAQHTFCKRCGVQSFYTPRSNPGGFGIAPHCLDEGTVRSMVTEEFNGSDWEKAMKEHKTIKNMSKE
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CENPV expression in transfected 293T cell line by CENPV monoclonal antibody (M05), clone 1D9.
Lane 1: CENPV transfected lysate (Predicted MW: 29.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CENPV is 1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to CENPV on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — CENPV
Entrez GeneID
201161GeneBank Accession#
NM_181716Protein Accession#
NP_859067.2Gene Name
CENPV
Gene Alias
3110013H01Rik, CENP-V, PRR6, p30
Gene Description
centromere protein V
Omim ID
608139Gene Ontology
HyperlinkOther Designations
nuclear protein p30|proline rich 6
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