MLKL monoclonal antibody (M02), clone 3B2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MLKL.
Immunogen
MLKL (NP_689862, 371 a.a. ~ 471 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
VKSTAYLSPQELEDVFYQYDVKSEIYSFGIVLWEIATGDIPFQGCNSEKIRKLVAVKRQQEPLGEDCPSELREIIDECRAHDPSVRPSVDEILKKLSTFSK
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.85 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to MLKL on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MLKL is 0.3 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to MLKL on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — MLKL
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Interactome
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Disease
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Publication Reference
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Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death.
Douglas T, Saleh M.
Cell Reports 2019 Dec; 29(11):3652.
Application:WB-Tr, Human, HaCaT cells.
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Antitumor activity of HPA3P through RIPK3-dependent regulated necrotic cell death in colon cancer.
Cho E, Lee JK, Park E, Seo CH, Luchian T, Park Y.
Oncotarget 2018 Jan; 9(8):7902.
Application:WB-Tr, Human, HCT-116 cells.
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Post-translational Modification of OTULIN Regulates Ubiquitin Dynamics and Cell Death.
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