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Last updated: 2017/12/17
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C9orf96 monoclonal antibody (M03), clone 2B5

  • Catalog # : H00169436-M03
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a partial recombinant C9orf96.
  • Immunogen:
  • C9orf96 (NP_714921, 1 a.a. ~ 99 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • MLGPGSNRRRPTQGERGPGSPGEPMEKYQVLYQLNPGALGVNLVVEEMETKVKHVIKQVECMDDHYASQALEELMPLLKLRHAHISVYQELFITWNGEI
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2a Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00169436-M03
    Western Blot detection against Immunogen (36.63 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of C9orf96 expression in transfected 293T cell line by C9orf96 monoclonal antibody (M03), clone 2B5.

    Lane 1: C9orf96 transfected lysate(75.5 KDa).
    Lane 2: Non-transfected lysate.
  • PDF DownloadProtocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged C9orf96 is approximately 0.03ng/ml as a capture antibody.
  • PDF DownloadProtocol Download
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • Western blot analysis of C9orf96 over-expressed 293 cell line, cotransfected with C9orf96 Validated Chimera RNAi ( Cat # H00169436-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with C9orf96 monoclonal antibody (M03), clone 2B5 (Cat # H00169436-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
  • PDF DownloadProtocol Download
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • RNAi Knockdown (Antibody validated)
  • RNAi Knockdown (Antibody validated)
  • enlarge
  • Gene Information
  • Gene Name:
  • C9orf96
  • Gene Alias:
  • MGC43306,RP11-244N20.8
  • Gene Description:
  • chromosome 9 open reading frame 96
  • Other Designations:
  • OTTHUMP00000022465,hypothetical protein LOC169436
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