RNF133 monoclonal antibody (M01), clone 3D6
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant RNF133.
Immunogen
RNF133 (NP_631914, 277 a.a. ~ 376 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
FFHKNCIDPWILPHGTCPICKCDILKVLGIQVVVENGTEPLQVLMSNELPETLSPSEEETNNEVSPAGTSDKVIHVEENPTSQNNDIQPHSVVEDVHPSP
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RNF133 expression in transfected 293T cell line by RNF133 monoclonal antibody (M01), clone 3D6.
Lane 1: RNF133 transfected lysate(42.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged RNF133 is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — RNF133
Entrez GeneID
168433GeneBank Accession#
NM_139175Protein Accession#
NP_631914Gene Name
RNF133
Gene Alias
MGC27072
Gene Description
ring finger protein 133
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RING finger domain, a motif present in a variety of functionally distinct proteins and known to be involved in protein-protein and protein-DNA interactions. This gene has no intron. [provided by RefSeq
Other Designations
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Interactome
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Disease
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