DDX53 (Human) Recombinant Protein (P01)
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More Files
- More Functions
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Specification
Product Description
Human DDX53 full-length ORF ( AAH67878.1, 1 a.a. - 631 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MSHWAPEWKRAEANPRDLGASWDVRGSRGSGWSGPFGHQGPRAAGSREPPLCFKIKNNMVGVVIGYSGSKIKDLQHSTNTKIQIINGESEAKVRIFGNREMKAKAKAAIETLIRKQESYNSESSVDNAASQTPIGRNLGRNDIVGEAEPLSNWDRIRAAVVECEKRKWADLPPVKKNFYIESKATSCMSEMQVINWRKENFNITCDDLKSGEKRLIPKPTCRFKDAFQQYPDLLKSIIRVGILKPTPIQSQAWPIILQGIDLIVVAQTGTGKTLSYLMPGFIHLDSQPISREQRNGPGMLVLTPTRELALHVEAECSKYSYKGLKSICIYGGRNRNGQIEDISKGVDIIIATPGRLNDLQMNNSVNLRSITYLVIDEADKMLDMEFEPQIRKILLDVRPDRQTVMTSATWPDTVRQLALSYLKDPMIVYVGNLNLVAVNTVKQNIIVTTEKEKRALTQEFVENMSPNDKVIMFVSQKHIADDLSSDFNIQGISAESLHGNSEQSDQERAVEDFKSGNIKILITTDIVSRGLDLNDVTHVYNYDFPRNIDVYVHRVGYIGRTGKTGTSVTLITQRDSKMAGELIKILDRANQSVPEDLVVMAEQYKLNQQKRHRETRSREPGQRRKEFYFLS
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
97.6
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — DDX53
Entrez GeneID
168400GeneBank Accession#
BC067878.1Protein Accession#
AAH67878.1Gene Name
DDX53
Gene Alias
CAGE
Gene Description
DEAD (Asp-Glu-Ala-Asp) box polypeptide 53
Gene Ontology
HyperlinkOther Designations
DEAD-box protein CAGE|cancer associated|cancer-associated
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Disease
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Publication Reference
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Disruption of DDX53 coding sequence has limited impact on iPSC-derived human NGN2 neurons.
Muhammad Faheem, Eric Deneault, Roumiana Alexandrova, Deivid C Rodrigues, Giovanna Pellecchia, Carole Shum, Mehdi Zarrei, Alina Piekna, Wei Wei, Jennifer L Howe, Bhooma Thiruvahindrapuram, Sylvia Lamoureux, P Joel Ross, Clarrisa A Bradley, James Ellis, Stephen W Scherer.
BMC Medical Genomics 2023 Jan; 16(1):5.
Application：WB-Re.
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miR-200b and cancer/testis antigen CAGE form a feedback loop to regulate a cancer cell line's invasion, tumorigenic, and angiogenic responses to microtubule-targeting drugs.
Kim Y, Park D, Kim H, Choi M, Lee H, Lee YS, Choe J, Kim YM, Jeoung D.
The Journal of Biological Chemistry 2013 Dec; 288(51):36502.
Application：WB-Ce, WB-Tr, Human, SNU387, SNU387R, SNU387R-Taxol, Malme3M, Malme3MR, Malme3MR-Taxol, AGS, AGSR, MCF-7, MDA-MB-231 cells, HUVECs.
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Disruption of DDX53 coding sequence has limited impact on iPSC-derived human NGN2 neurons.
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