Western blot analysis of MGC26963 over-expressed 293 cell line, cotransfected with MGC26963 Validated Chimera RNAi ( Cat # H00166929-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MGC26963 monoclonal antibody (M08), clone 7D10 (Cat # H00166929-M08 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sphingomyelin, a major component of cell and Golgi membranes, is made by the transfer of phosphocholine from phosphatidylcholine onto ceramide, with diacylglycerol as a side product. The protein encoded by this gene is an enzyme that catalyzes this reaction primarily at the cell membrane. The synthesis is reversible, and this enzyme can catalyze the reaction in either direction. The encoded protein is required for cell growth. Three transcript variants encoding the same protein have been found for this gene. There is evidence for more variants, but the full-length nature of their transcripts has not been determined