RNF168 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RNF168 transfected lysate.
Western Blot
Lane 1: RNF168 transfected lysate ( 62.92 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RNF168 full-length
Host
Human
Theoretical MW (kDa)
62.92
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RNF168 antibody (H00165918-B01) by Western Blots.
SDS-PAGE Gel
RNF168 transfected lysate.
Western Blot
Lane 1: RNF168 transfected lysate ( 62.92 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RNF168
Entrez GeneID
165918GeneBank Accession#
NM_152617.2Protein Accession#
NP_689830.2Gene Name
RNF168
Gene Alias
FLJ35794
Gene Description
ring finger protein 168
Gene Ontology
HyperlinkGene Summary
The complex repair response elicited by DNA double-strand breaks (DSBs) includes recruitment of several DNA repair proteins and ubiquitination of H2A-type histones (see MIM 142720). RNF168 is an E3 ubiquitin ligase critical for DSB repair (Stewart et al., 2009 [PubMed 19203578]).[supplied by OMIM
Other Designations
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