This antibody pair set comes with matched antibody pair to detect and quantify protein level of human RNF168.
Quality Control Testing:
Standard curve using RNF168 293T overexpression lysate (non-denatured) as an analyte.
Sandwich ELISA detection sensitivity ranging from approximately 729x to 3x dilution of the RNF168 293T overexpression lysate (non-denatured).
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-RNF168, IgG2b Kappa (100 ug) 2. Detection antibody: rabbit purified polyclonal anti-RNF168 (50 ug) *Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
The complex repair response elicited by DNA double-strand breaks (DSBs) includes recruitment of several DNA repair proteins and ubiquitination of H2A-type histones (see MIM 142720). RNF168 is an E3 ubiquitin ligase critical for DSB repair (Stewart et al., 2009 [PubMed 19203578]).[supplied by OMIM