HSFY2 monoclonal antibody (M02), clone 4B7
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant HSFY2.
Immunogen
HSFY2 (NP_714927.1, 1 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAHVSSETQDVSPKDELTASEASTRSPLCEHTFPGDSDLRSMIEEHAFQVLSQGSLLESPSYTVCVSEPDKDDDFLSLNFPRKLWKIVESDQFKSISWDE
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of HSFY2 expression in transfected 293T cell line by HSFY2 monoclonal antibody (M02), clone 4B7.
Lane 1: HSFY2 transfected lysate (Predicted MW: 23.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged HSFY2 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to HSFY2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — HSFY2
Entrez GeneID
159119GeneBank Accession#
NM_153716Protein Accession#
NP_714927.1Gene Name
HSFY2
Gene Alias
FLJ25453, HSF2L, HSFY
Gene Description
heat shock transcription factor, Y linked 2
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the heat shock factor (HSF) family of transcriptional activators for heat shock proteins. This gene is a candidate gene for azoospermia, since it localizes to a region of chromosome Y that is sometimes deleted in infertile males. The genome has two identical copies of this gene within a palindromic region; this record represents the more telomeric copy. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000038975|heat shock transcription factor 2-like
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