AMOT 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
AMOT transfected lysate.
Western Blot
Lane 1: AMOT transfected lysate ( 72.50 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-AMOT full-length
Host
Human
Theoretical MW (kDa)
72.5
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-AMOT antibody (H00154796-B01) by Western Blots.
SDS-PAGE Gel
AMOT transfected lysate.
Western Blot
Lane 1: AMOT transfected lysate ( 72.50 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — AMOT
Entrez GeneID
154796GeneBank Accession#
NM_133265.2Protein Accession#
NP_573572.1Gene Name
AMOT
Gene Alias
KIAA1071
Gene Description
angiomotin
Omim ID
300410Gene Ontology
HyperlinkGene Summary
This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
OTTHUMP00000023866|angiomotin p130 isoform|angiomotin p80 isoform
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Interactome
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Disease
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