SH3RF2 monoclonal antibody (M01), clone 4E10
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant SH3RF2.
Immunogen
SH3RF2 (NP_660205, 640 a.a. ~ 729 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
VKTVRFQNYSPPPTKHYTSHPTSGKPEQPATLKASQPEAASLGPEMTVLFAHRSGCHSGQQTDLRRKSALAKATTLVSTASGTQTVFPSK
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.64 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SH3RF2 is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — SH3RF2
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Interactome
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Disease
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Publication Reference
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SH3RF2 Functions as an Oncogene by Mediating PAK4 Protein Stability.
Kim TW, Kang YK, Park ZY, Kim YH, Hong SW, Su JO, Sohn HA, Yang SJ, Jang YJ, Lee DC, Kim SY, Yoo HS, Kim E, Yeom YI, Park KC.
Carcinogenesis 2014 Mar; 35(3):624.
Application:WB-Ce, WB-Tr, Human, SW620, SW480, HT29, Hep3B, HepG2, SK-Hep1, PLC/PRF/5, HEK 293T, HeLa, IMR90, Huh7 cells.
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Sh3rf2/POSHER promotes cell survival by RING-mediated proteasomal degradation of the JNK scaffold POSH.
Wilhelm M, Kukekov NV, Schmit TL, Biagas KV, Sproul AA, Gire S, Maes ME, Xu Z, Greene LA.
The Journal of Biological Chemistry 2012 Jan; 287(3):2247.
Application:WB, Human, Rat, HEK 293, C6 glioma cells.
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SH3RF2 Functions as an Oncogene by Mediating PAK4 Protein Stability.
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