SEPT10 monoclonal antibody (M01), clone 2A12
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant SEPT10.
Immunogen
SEPT10 (AAH20502, 1 a.a. ~ 454 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MASSEVARHLLFQSHMATKTTCMSSQGSDDEQIKRENIRSLTMSGHVGFESLPDQLVNRSIQQGFCFNILCVGETGIGKSTLIDTLFNTNFEDYESSHFCPNVKLKAQTYELQESNVQLKLTIVNTVGFGDQINKEESYQPIVDYIDAQFEAYLQEELKIKRSLFTYHDSRIHVCLYFISPTGHSLKTLDLLTMKNLDSKVNIIPVIAKADTVSKTELQKFKIKLMSELVSNGVQIYQFPTDDDTIAKVNAAMNGQLPFAVVGSMDEVKVGNKMVKARQYPWGVVQVENENHCDFVKLREMLICTNMEDLREQTHTRHYELYRRCKLEEMGFTDVGPENKPVSVQETYEAKRHEFHGERQRKEEEMKQMFVQRVKEKEAILKEAERELQAKFEHLKRLHQEERMKLEEKRRLLEEEIIAFSKKKATSEIFHSQSFLATGSNLRKDKDRKNSNFL
Host
Mouse
Reactivity
Human
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (75.68 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
SEPT10 monoclonal antibody (M01), clone 2A12 Western Blot analysis of SEPT10 expression in U-2 OS ( Cat # L022V1 ).Western Blot (Transfected lysate)
Western Blot analysis of SEPT10 expression in transfected 293T cell line by SEPT10 monoclonal antibody (M01), clone 2A12.
Lane 1: SEPT10 transfected lysate(52.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SEPT10 is approximately 1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SEPT10 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — SEPT10
Entrez GeneID
151011GeneBank Accession#
BC020502Protein Accession#
AAH20502Gene Name
SEPT10
Gene Alias
FLJ11619
Gene Description
septin 10
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the septin family of cytoskeletal proteins with GTPase activity. This protein localizes to the cytoplasm and nucleus and displays GTP-binding and GTPase activity. Alternate splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
sept1-like
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Interactome
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Publication Reference
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Identification of a novel role of Septin 10 in paclitaxel-resistance in cancers through a functional genomics screen.
Xu M, Takanashi M, Oikawa K, Nishi H, Isaka K, Yoshimoto T, Ohyashiki J, Kuroda M.
Cancer Science 2012 Apr; 103(4):821.
Application:IHC-P, WB-Tr, Human, HeLa, MCF-7 cells, Human ovarian carcinoma specimens.
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Identification of a novel role of Septin 10 in paclitaxel-resistance in cancers through a functional genomics screen.
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