EME1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
EME1 transfected lysate.
Western Blot
Lane 1: EME1 transfected lysate ( 64.24 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-EME1 full-length
Host
Human
Theoretical MW (kDa)
64.24
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-EME1 antibody (H00146956-B01) by Western Blots.
SDS-PAGE Gel
EME1 transfected lysate.
Western Blot
Lane 1: EME1 transfected lysate ( 64.24 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — EME1
Entrez GeneID
146956GeneBank Accession#
BC016470.2Protein Accession#
AAH16470.1Gene Name
EME1
Gene Alias
FLJ31364, MMS4L
Gene Description
essential meiotic endonuclease 1 homolog 1 (S. pombe)
Omim ID
610885Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that complexes with methyl methanesulfonate-sensitive UV-sensitive 81 protein to form an endonuclease complex. The encoded protein interacts with specifc DNA structures including nicked Holliday junctions, 3'-flap structures and aberrant replication fork structures. This protein may be involved in repairing DNA damage and in maintaining genomic stability
Other Designations
essential meiotic endonuclease 1 homolog 1|essential meiotic endonuclease 1 homolog 2|homolog of yeast EME1 endonuclease
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Interactome
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Pathway
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Disease
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