ASB9 monoclonal antibody (M01), clone 1D8
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant ASB9.
Immunogen
ASB9 (AAH01244, 1 a.a. ~ 252 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDGKQGGMDGSKPAGPRDFPGIRLLSNPLMGDAVSDWSPMHEAAIHGHQLSLRNLISQGWAVNIITADHVSPLHEACLGGHLSCVKILLKHGAQVNGVTADWHTPLFNACVSGSWDCVNLLLQHGASVQPESDLASPIHEAARRAYGGNIDHKISHLGTPLYLACENQQRACVKKLLESGADVNQGKGQDSPLHAVARTASEELACLLMDFGADTQAKNAEGKRPVELVPPESPLAQLFLEREGASLPKPKP
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (53.46 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ASB9 monoclonal antibody (M01), clone 1D8 Western Blot analysis of ASB9 expression in HepG2 ( Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of ASB9 expression in transfected 293T cell line by ASB9 monoclonal antibody (M01), clone 1D8.
Lane 1: ASB9 transfected lysate(31.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to ASB9 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3 ug/ml]Immunoprecipitation
Immunoprecipitation of ASB9 transfected lysate using anti-ASB9 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ASB9 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ASB9 is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — ASB9
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Interactome
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Publication Reference
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ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function.
Kwon S, Kim D, Rhee JW, Park JA, Kim DW, Kim DS, Lee Y, Kwon HJ.
BMC Biology 2010 Mar; 8:23.
Application:IP, WB-Ce, Human, HEK 293, Huh-7 cells, Human hepatocytes, PBMCs.
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ASB9 interacts with ubiquitous mitochondrial creatine kinase and inhibits mitochondrial function.
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