ADHFE1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
ADHFE1 transfected lysate.
Western Blot
Lane 1: ADHFE1 transfected lysate ( 46.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ADHFE1 full-length
Host
Human
Theoretical MW (kDa)
46.2
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ADHFE1 antibody (H00137872-B01) by Western Blots.
SDS-PAGE Gel
ADHFE1 transfected lysate.
Western Blot
Lane 1: ADHFE1 transfected lysate ( 46.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ADHFE1
Entrez GeneID
137872GeneBank Accession#
NM_144650.1Protein Accession#
NP_653251.1Gene Name
ADHFE1
Gene Alias
ADH8, FLJ32430, HMFT2263, HOT, MGC48605
Gene Description
alcohol dehydrogenase, iron containing, 1
Omim ID
611083Gene Ontology
HyperlinkGene Summary
The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al., 2006 [PubMed 16616524]).[supplied by OMIM
Other Designations
Fe-containing alcohol dehydrogenase 1|alcohol dehydrogenase 8|hydroxyacid-oxoacid transhydrogenase
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