ADHFE1 (Human) Recombinant Protein (P01)
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Specification
Product Description
Human ADHFE1 full-length ORF ( NP_653251.1, 1 a.a. - 419 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MAVSNIRYGAAVTKEVGMDLKNMGAKNVCLMTDKNLSKLPPVQVAMDSLVKNGIPFTVYDNVRVEPTDSSFMEAIEFAQKGAFDAYVAVGGGSTMDTCKAANLYASSPHSDFLDYVSAPIGKGKPVSVPLKPLIAVPTTSGTGSETTGVAIFDYEHLKVKIGITSRAIKPTLGLIDPLHTLHMPARVVANSGFDVLCHALESYTTLPYHLRSPCPSNPITRPAYQGSNPISDIWAIHALRIVAKYLKRAVRNPDDLEARSHMHLASAFAGIGFGNAGVHLCHGMSYPISGLVKMYKAKDYNVDHPLVPHGLSVVLTSPAVFTFTAQMFPERHLEMAEILGADTRTARIQDAGLVLADTLRKFLFDLDVDDGLAAVGYSKADIPALVKGTLPQERVTKLAPRPQSEEDLAALFEASMKLY
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
71.6
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — ADHFE1
Entrez GeneID
137872GeneBank Accession#
NM_144650.1Protein Accession#
NP_653251.1Gene Name
ADHFE1
Gene Alias
ADH8, FLJ32430, HMFT2263, HOT, MGC48605
Gene Description
alcohol dehydrogenase, iron containing, 1
Omim ID
611083Gene Ontology
HyperlinkGene Summary
The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase (EC 1.1.99.24), which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues (Kardon et al., 2006 [PubMed 16616524]).[supplied by OMIM
Other Designations
Fe-containing alcohol dehydrogenase 1|alcohol dehydrogenase 8|hydroxyacid-oxoacid transhydrogenase
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