GPR175 monoclonal antibody (M01), clone 6D7
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant GPR175.
Immunogen
GPR175 (NP_057456, 281 a.a. ~ 371 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
RGFFGSEPKILFSYKCQVDETEEPDVHLPQPYAVARREGLEAAGAAGASAASYSSTQFDSAGGVAYLDDIASMPCHTGSINSTDSERWKAI
Host
Mouse
Reactivity
Human
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.75 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of GPR175 expression in transfected 293T cell line by GPR175 monoclonal antibody (M01), clone 6D7.
Lane 1: GPR175 transfected lysate(41.053 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of GPR175 over-expressed 293 cell line, cotransfected with GPR175 Validated Chimera RNAi ( Cat # H00131601-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR175 monoclonal antibody (M01), clone 6D7 (Cat # H00131601-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — GPR175
Entrez GeneID
131601GeneBank Accession#
NM_016372Protein Accession#
NP_057456Gene Name
GPR175
Gene Alias
FLJ32197, TPRA40
Gene Description
G protein-coupled receptor 175
Omim ID
608336Gene Ontology
HyperlinkOther Designations
seven transmembrane domain orphan receptor|transmembrane domain protein regulated in adipocytes 40 kDa
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