TBN 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TAF8 transfected lysate.
Western Blot
Lane 1: TAF8 transfected lysate ( 18.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TBN full-length
Host
Human
Theoretical MW (kDa)
18.7
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TBN antibody (H00129685-B01P) by Western Blots.
SDS-PAGE Gel
TAF8 transfected lysate.
Western Blot
Lane 1: TAF8 transfected lysate ( 18.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TAF8
Entrez GeneID
129685GeneBank Accession#
BC033728Protein Accession#
AAH33728.1Gene Name
TAF8
Gene Alias
43, FLJ32821, II, TAF, TAFII43, TBN
Gene Description
TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 43kDa
Omim ID
609514Gene Ontology
HyperlinkGene Summary
This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq
Other Designations
OTTHUMP00000016392|TAF(II)43|TAF8 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 50 kD|TATA box binding protein (TBP)-associated factor, RNA polymerase II, A, 45/50kDa|TBP-associated factor 8|TBP-associated factor TAFII43|TBP-associa
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Interactome
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Disease
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