ATP6V1G3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ATP6V1G3 full-length
Host
Human
Theoretical MW (kDa)
13.09
Interspecies Antigen Sequence
Mouse (84); Rat (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ATP6V1G3 antibody (H00127124-B01) by Western Blots.
SDS-PAGE Gel
ATP6V1G3 transfected lysate.
Western Blot
Lane 1: ATP6V1G3 transfected lysate ( 13.09 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — ATP6V1G3
Entrez GeneID
127124GeneBank Accession#
NM_133262.2Protein Accession#
NP_573569.1Gene Name
ATP6V1G3
Gene Alias
ATP6G3, MGC119810, MGC119813, Vma10
Gene Description
ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3
Gene Ontology
HyperlinkGene Summary
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ATPase, H+ transporting, lysosomal (vacuolar proton pump) subunit G3|ATPase, H+ transporting, lysosomal 13kD, V1 subunit G|ATPase, H+ transporting, lysosomal, V1 subunit G3|OTTHUMP00000033686|V-ATPase 13 kDa subunit 3|V-ATPase G subunit 3|V-ATPase G3 subu
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