ATP6V1G3 monoclonal antibody (M13), clone 3A5
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ATP6V1G3.
Immunogen
ATP6V1G3 (NP_573569, 38 a.a. ~ 118 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EEAMVEIDQYRMQRDKEFRLKQSKIMGSQNNLSDEIEEQTLGKIQELNGHYNKYMESVMNQLLSMVCDMKPEIHVNYRATN
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (80); Rat (77)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (34.65 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ATP6V1G3 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — ATP6V1G3
Entrez GeneID
127124GeneBank Accession#
NM_133262Protein Accession#
NP_573569Gene Name
ATP6V1G3
Gene Alias
ATP6G3, MGC119810, MGC119813, Vma10
Gene Description
ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3
Gene Ontology
HyperlinkGene Summary
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ATPase, H+ transporting, lysosomal (vacuolar proton pump) subunit G3|ATPase, H+ transporting, lysosomal 13kD, V1 subunit G|ATPase, H+ transporting, lysosomal, V1 subunit G3|OTTHUMP00000033686|V-ATPase 13 kDa subunit 3|V-ATPase G subunit 3|V-ATPase G3 subu
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