ATP6V1G3 rabbit monoclonal antibody
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Specification
Product Description
Rabbit monoclonal antibody raised against a human ATP6V1G3 peptide using ARM Technology.
Immunogen
A synthetic peptide of human ATP6V1G3 is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human ATP6V1G3 peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — ATP6V1G3
Entrez GeneID
127124GeneBank Accession#
ATP6V1G3Gene Name
ATP6V1G3
Gene Alias
ATP6G3, MGC119810, MGC119813, Vma10
Gene Description
ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G3
Gene Ontology
HyperlinkGene Summary
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'' and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene encodes one of three G subunit proteins. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
ATPase, H+ transporting, lysosomal (vacuolar proton pump) subunit G3|ATPase, H+ transporting, lysosomal 13kD, V1 subunit G|ATPase, H+ transporting, lysosomal, V1 subunit G3|OTTHUMP00000033686|V-ATPase 13 kDa subunit 3|V-ATPase G subunit 3|V-ATPase G3 subu
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Pathway
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