MSI2 monoclonal antibody (M11), clone 3B6
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of MSI2 expression in transfected 293T cell line by MSI2 monoclonal antibody (M11), clone 3B6.
Lane 1: MSI2 transfected lysate(35.2 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of MSI2 transfected lysate using anti-MSI2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MSI2 monoclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MSI2 is 0.1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (61.82 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant MSI2.
Immunogen
MSI2 (AAH01526, 1 a.a. ~ 328 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEANGSQGTSGSANDSQHDPGKMFIGGLSWQTSPDSLRDYFSKFGEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQPKMVTRTKKIFVGGLSANTVVEDVKQYFEQFGKVEDAMLMFDKTTNRHRGFGFVTFENEDVVEKVCEIHFHEINNKMVECKKAQPKEVMFPPGTRGRARGLPYTMDAFMLGMGMLGYPNFVATYGRGYPGFAPSYGYQFPGFPAAAYGPVAAAAVAAARGSGSNPARPGGFPGANSPGPVADLYGPASQDSGVGNYISAASPQPGSGFGHGIAGPLIATAFTNGYH
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (61.82 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MSI2 expression in transfected 293T cell line by MSI2 monoclonal antibody (M11), clone 3B6.
Lane 1: MSI2 transfected lysate(35.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of MSI2 transfected lysate using anti-MSI2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MSI2 monoclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MSI2 is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — MSI2
Entrez GeneID
124540GeneBank Accession#
BC001526Protein Accession#
AAH01526Gene Name
MSI2
Gene Alias
FLJ36569, MGC3245, MSI2H
Gene Description
musashi homolog 2 (Drosophila)
Omim ID
607897Gene Ontology
HyperlinkGene Summary
This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
musashi 2
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Interactome
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Disease
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Publication Reference
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Msi2-mediated MiR7a-1 processing repression promotes myogenesis.
Wenjun Yang, Lele Yang, Jianhua Wang, Yuanyuan Zhang, Sheng Li, Qi Yin, Jinlong Suo, Ruimiao Ma, Yuzhen Ye, Hong Cheng, Jinsong Li, Jingyi Hui, Ping Hu.
Journal of Cachexia, Sarcopenia and Muscle 2022 Feb; 13(1):728.
Application：WB-Ce, WB-Tr, Mouse, Mouse muscle stem cells (Mouse muscle).
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Msi2-mediated MiR7a-1 processing repression promotes myogenesis.
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