SENP8 (Human) IP-WB Antibody Pair
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More Files
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Immunoprecipitation of SENP8 transfected lysate using rabbit polyclonal anti-SENP8 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse monoclonal anti-SENP8.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Interspecies Antigen Sequence
Mouse (92); Rat (92)
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of SENP8 transfected lysate using rabbit polyclonal anti-SENP8 and Protein A Magnetic Bead (U0007), and immunoblotted with mouse monoclonal anti-SENP8.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-SENP8 (300 ul)
2. Antibody pair for WB: mouse monoclonal anti-SENP8 (50 ug)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — SENP8
Entrez GeneID
123228Gene Name
SENP8
Gene Alias
DEN1, HsT17512, NEDP1, PRSC2
Gene Description
SUMO/sentrin specific peptidase family member 8
Omim ID
608659Gene Ontology
HyperlinkGene Summary
NEDD8 (MIM 603171) is a ubiquitin-like protein that becomes conjugated to the cullin (see CUL1; MIM 603134) subunit of several ubiquitin ligases. This conjugation, called neddylation, is required for optimal ubiquitin ligase activity. NEDD8-specific deneddylases, such as NEDP1, or DEN1, are required to process the NEDD8 propeptide at a C-terminal diglycine motif and to remove NEDD8 from cullins (Gan-Erdene et al., 2003 [PubMed 12759363]).[supplied by OMIM
Other Designations
NEDD8-specific protease 1|SUMO/sentrin specific protease family member 8|deneddylase 1|protease, cysteine, 2 (NEDD8 specific)|sentrin/SUMO-specific protease SENP8
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