UBE2J2 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
UBE2J2 transfected lysate.
Western Blot
Lane 1: UBE2J2 transfected lysate ( 28.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-UBE2J2 full-length
Host
Human
Theoretical MW (kDa)
28.6
Interspecies Antigen Sequence
Mouse (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-UBE2J2 antibody (H00118424-B01) by Western Blots.
SDS-PAGE Gel
UBE2J2 transfected lysate.
Western Blot
Lane 1: UBE2J2 transfected lysate ( 28.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — UBE2J2
Entrez GeneID
118424GeneBank Accession#
NM_058167.2Protein Accession#
NP_477515.2Gene Name
UBE2J2
Gene Alias
NCUBE2, PRO2121
Gene Description
ubiquitin-conjugating enzyme E2, J2 (UBC6 homolog, yeast)
Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq
Other Designations
OTTHUMP00000001907|OTTHUMP00000002078|ubiquitin conjugating enzyme 6|ubiquitin conjugating enzyme E2, J2|yeast UBC6 homolog
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Interactome
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