TWIST2 monoclonal antibody (M06), clone 1E2
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant TWIST2.
Immunogen
TWIST2 (AAH33168, 1 a.a. ~ 160 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEEGSSSPVSPVDSLGTSEEELERQPKRFGRKRRYSKKSSEDGSPTPGKRGKKGSPSAQSFEELQSQRILANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDEMDNKMTSCSYVAHERLSYAFSVWRMEGSWSMSASH
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100); Rat (100)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (43.34 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of TWIST2 expression in transfected 293T cell line by TWIST2 monoclonal antibody (M06), clone 1E2.
Lane 1: TWIST2 transfected lysate(18.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of TWIST2 transfected lysate using anti-TWIST2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TWIST2 MaxPab rabbit polyclonal antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to TWIST2 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — TWIST2
Entrez GeneID
117581GeneBank Accession#
BC033168Protein Accession#
AAH33168Gene Name
TWIST2
Gene Alias
DERMO1, MGC117334, bHLHa39
Gene Description
twist homolog 2 (Drosophila)
Omim ID
607556Gene Ontology
HyperlinkGene Summary
Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Twist. It is thought that during osteoblast development this protein may inhibit osteoblast maturation and maintain cells in a preosteoblast phenotype. [provided by RefSeq
Other Designations
dermis-expressed protein 1|twist homolog 2|twist-related bHLH protein Dermo1
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