RASGRP4 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RASGRP4 full-length
Host
Human
Theoretical MW (kDa)
74.14
Interspecies Antigen Sequence
Mouse (85); Rat (82)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RASGRP4 antibody (H00115727-B01) by Western Blots.
SDS-PAGE Gel
RASGRP4 transfected lysate.
Western Blot
Lane 1: RASGRP4 transfected lysate ( 74.14 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RASGRP4
Entrez GeneID
115727GeneBank Accession#
NM_170603Protein Accession#
NP_733748Gene Name
RASGRP4
Gene Alias
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Gene Description
RAS guanyl releasing protein 4
Omim ID
607320Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
guanyl nucleotide releasing protein 4
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Interactome
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