Products

Western Blot (Transfected lysate)

Western Blot analysis of RASGRP4 expression in transfected 293T cell line (H00115727-T01) by RASGRP4 MaxPab polyclonal antibody.

Lane 1: RASGRP4 transfected lysate(74.90 KDa).
Lane 2: Non-transfected lysate.

In situ Proximity Ligation Assay (Cell)

Proximity Ligation Analysis of protein-protein interactions between RASGRP4 and RAP1A. HeLa cells were stained with anti-RASGRP4 rabbit purified polyclonal 1:1200 and anti-RAP1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Entrez GeneID

115727

GeneBank Accession#

NM_170603

Protein Accession#

NP_733749.1

Gene Name

RASGRP4

Gene Alias

-

Gene Description

RAS guanyl releasing protein 4

Omim ID

607320

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq

Other Designations

guanyl nucleotide releasing protein 4