TOE1 monoclonal antibody (M01), clone 1D8
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant TOE1.
Immunogen
TOE1 (AAH09364, 1 a.a. ~ 510 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAADSDDGAVSAPAASDGGVSKSTTSGEELVVQVPVVDVQSNNFKEMWPSLLLAIKTANFVAVDTELSGLGDRKSLLNQCIEERYKAVCHAARTRSILSLGLACFKRQPDKGEHSYLAQVFNLTLLCMEEYVIEPKSVQFLIQHGFNFNQQYAQGIPYHKGNDKGDESQSQSVRTLFLELIRARRPLVLHNGLIDLVFLYQNFYAHLPESLGTFTADLCEMFPAGIYDTKYAAEFHARFVASYLEYAFRKCERENGKQRAAGSPHLTLEFCNYPSSMRDHIDYRCCLPPATHRPHPTSICDNFSAYGWCPLGPQCPQSHDIDLIIDTDEAAAEDKRRRRRRREKRKRALLNLPGTQTSGEAKDGPPKKQVCGDSIKPEETEQEVAADETRNLPHSKQGNKNDLEMGIKAARPEIADRATSEVPGSQASPNPVPGDGLHRAGFDAFMTGYVMAYVEVSQGPQPCSSGPWLPECHNKVYLSGKAVPLTVAKSQFSRSSKAHNQKMKLTWGSS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (81); Rat (85)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (81.84 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
TOE1 monoclonal antibody (M01), clone 1D8 Western Blot analysis of TOE1 expression in Jurkat ( Cat # L017V1 ).Western Blot (Transfected lysate)
Western Blot analysis of TOE1 expression in transfected 293T cell line by TOE1 monoclonal antibody (M01), clone 1D8.
Lane 1: TOE1 transfected lysate(56.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to TOE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.3 ug/ml]ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of TOE1 over-expressed 293 cell line, cotransfected with TOE1 Validated Chimera RNAi ( Cat # H00114034-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TOE1 monoclonal antibody (M01), clone 1D8 (Cat # H00114034-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — TOE1
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Interactome
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Publication Reference
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TOE1 is an inhibitor of HIV-1 replication with cell-penetrating capability.
Sperandio S, Barat C, Cabrita MA, Gargaun A, Berezovski MV, Tremblay MJ, de Belle I.
Proceedings of the National Academy of Sciences of the United States of America 2015 Jun; 112(26):E3392.
Application:WB, Human, T cells.
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TOE1 interacts with p53 to modulate its transactivation potential.
Sperandio S, Tardito S, Surzycki A, Latterich M, de Belle I.
FEBS Letters 2009 Jul; 583(13):2165.
Application:WB-Tr, WB-Re, Human, HEK 293 cells, Recombinant protein.
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TOE1 is an inhibitor of HIV-1 replication with cell-penetrating capability.
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