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Last updated: 2016/11/27

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MED8 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00112950-T01
  • Visit Frequency :
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-MED8 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 23.43
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-MED8 antibody (H00112950-B01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of H00112950-T01
    MED8 transfected lysate.
    Western Blot
    QC Testing of H00112950-T01
    Lane 1: MED8 transfected lysate ( 23.43 KDa)
    Lane 2: Non-transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Protein Accession#:
  • -
  • Gene Name:
  • MED8
  • Gene Alias:
  • ARC32,MGC17544,MGC19641
  • Gene Description:
  • mediator complex subunit 8
  • Gene Summary:
  • This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000008573,OTTHUMP00000008581,activator-recruited cofactor 32 kDa component,mediator of RNA polymerase II transcription subunit MED8,mediator of RNA polymerase II transcription, subunit 8 homolog
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