PPP1R14A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PPP1R14A full-length
Host
Human
Theoretical MW (kDa)
16.7
Interspecies Antigen Sequence
Mouse (85); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PPP1R14A antibody (H00094274-D01P) by Western Blots.
SDS-PAGE Gel
PPP1R14A transfected lysate.
Western Blot
Lane 1: PPP1R14A transfected lysate ( 16.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PPP1R14A
Entrez GeneID
94274GeneBank Accession#
NM_033256.1Protein Accession#
NP_150281.1Gene Name
PPP1R14A
Gene Alias
CPI-17, CPI17, PPP1INL
Gene Description
protein phosphatase 1, regulatory (inhibitor) subunit 14A
Omim ID
608153Gene Ontology
HyperlinkGene Summary
PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase (see MIM 603768). Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration.[supplied by OMIM
Other Designations
17-KDa protein|17-kDa PKC-potentiated inhibitory protein of PP1|PKC-potentiated inhibitory protein of PP1
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Interactome
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Pathway
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