NAPRT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
NAPRT1 transfected lysate.
Western Blot
Lane 1: NAPRT1 transfected lysate ( 49.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-NAPRT1 full-length
Host
Human
Theoretical MW (kDa)
49.6
Interspecies Antigen Sequence
Mouse (87)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-NAPRT1 antibody (H00093100-B01) by Western Blots.
SDS-PAGE Gel
NAPRT1 transfected lysate.
Western Blot
Lane 1: NAPRT1 transfected lysate ( 49.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — NAPRT1
Entrez GeneID
93100GeneBank Accession#
NM_145201Protein Accession#
NP_660202Gene Name
NAPRT1
Gene Alias
PP3856
Gene Description
nicotinate phosphoribosyltransferase domain containing 1
Omim ID
611552Gene Ontology
HyperlinkGene Summary
Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM
Other Designations
nicotinic acid phosphoribosyltransferase
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