RFT1 rabbit monoclonal antibody
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Specification
Product Description
Rabbit monoclonal antibody raised against a human RFT1 peptide using ARM Technology.
Immunogen
A synthetic peptide of human RFT1 is used for rabbit immunization.
Customer or Abnova will decide on the preferred peptide sequence.Host
Rabbit
Library Construction
Non-fusion antibody library from rabbit spleen (ARM Technology).
Expression
Overexpression vector and transfection into 293H cell line.
Reactivity
Human
Purification
Protein A
Isotype
IgG
Quality Control Testing
Antibody reactive against human RFT1 peptide by ELISA and mammalian transfected lysate by Western Blot.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Deliverable
Up to three rabbit IgG clones of 100 ug each will be delivered to customer.
Note
1. Customer may provide cell or tissue lysate for antibody screening.
2. Rabbit monoclonal antibody generated by ARM technology is amenable to antibody engineering including F(ab)2, IgG, scFv and different Fc and non-Fc conjugates per customer request. -
Applications
Western Blot (Transfected lysate)
ELISA
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Gene Info — RFT1
Entrez GeneID
91869GeneBank Accession#
RFT1Gene Name
RFT1
Gene Alias
CDG1N, DKFZp667J092, FLJ25945
Gene Description
RFT1 homolog (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
This gene encodes an enzyme which catalyzes the translocation of the Man(5)GlcNAc (2)-PP-Dol intermediate from the cytoplasmic to the luminal side of the endoplasmic reticulum membrane in the pathway for the N-glycosylation of proteins. Mutations in this gene are associated with congenital disorder of glycosylation type In
Other Designations
RFT1 homolog|RFT1, requiring fifty three 1 homolog|putative endoplasmic reticulum multispan transmembrane protein
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