IFT20 monoclonal antibody (M02), clone 3F3
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant IFT20.
Immunogen
IFT20 (NP_777547.1, 1 a.a. ~ 148 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MAKDILGEAGLHFDELNKLRVLDPEVTQQTIELKEECKDFVDKIGQFQKIVGGLIELVDQLAKEAENEKMKSLAVSPRLECTGAISAHCKLCLSDSSDSPTSPSRVGGTTGHRCSELAQIYSKAERSSTAATSSPNSRKENAARKVSG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (42.4 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of IFT20 expression in transfected 293T cell line by IFT20 monoclonal antibody (M02), clone 3F3.
Lane 1: IFT20 transfected lysate(16 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged IFT20 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to IFT20 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — IFT20
Entrez GeneID
90410GeneBank Accession#
NM_174887.2Protein Accession#
NP_777547.1Gene Name
IFT20
Gene Alias
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Gene Description
intraflagellar transport 20 homolog (Chlamydomonas)
Gene Ontology
HyperlinkOther Designations
intraflagellar transport protein 20-like|intraflagellar transport protein IFT20
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Interactome
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