PNPT1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PNPT1 transfected lysate.
Western Blot
Lane 1: PNPT1 transfected lysate ( 85.9 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PNPT1 full-length
Host
Human
Theoretical MW (kDa)
85.9
Interspecies Antigen Sequence
Mouse (91); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PNPT1 antibody (H00087178-D01P) by Western Blots.
SDS-PAGE Gel
PNPT1 transfected lysate.
Western Blot
Lane 1: PNPT1 transfected lysate ( 85.9 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PNPT1
Entrez GeneID
87178GeneBank Accession#
NM_033109.2Protein Accession#
NP_149100.1Gene Name
PNPT1
Gene Alias
DKFZp762K1914, OLD35, PNPASE, old-35
Gene Description
polyribonucleotide nucleotidyltransferase 1
Omim ID
610316Gene Ontology
HyperlinkGene Summary
PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM
Other Designations
3'-5' RNA exonuclease|polynucleotide phosphorylase|polynucleotide phosphorylase-like
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