CCNB3 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CCNB3 transfected lysate.
Western Blot
Lane 1: CCNB3 transfected lysate ( 12.2 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CCNB3 full-length
Host
Human
Theoretical MW (kDa)
12.2
Interspecies Antigen Sequence
Mouse (47); Rat (44)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CCNB3 antibody (H00085417-B01) by Western Blots.
SDS-PAGE Gel
CCNB3 transfected lysate.
Western Blot
Lane 1: CCNB3 transfected lysate ( 12.2 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CCNB3
Entrez GeneID
85417GeneBank Accession#
ENST00000327388Protein Accession#
ENSP00000365204Gene Name
CCNB3
Gene Alias
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Gene Description
cyclin B3
Omim ID
300456Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Studies of similar genes in chick and Drosophila suggest that this cyclin may associate with CDC2 and CDK2 kinases, and be required for proper spindle reorganization and restoration of the interphase nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000023292|OTTHUMP00000023293
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