MYLK2 monoclonal antibody (M01), clone 2G1
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of MYLK2 expression in transfected 293T cell line by MYLK2 monoclonal antibody (M01), clone 2G1.
Lane 1: MYLK2 transfected lysate(64.7 KDa).
Lane 2: Non-transfected lysate.
RNAi Knockdown (Antibody validated)
Western blot analysis of MYLK2 over-expressed 293 cell line, cotransfected with MYLK2 Validated Chimera RNAi ( Cat # H00085366-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYLK2 monoclonal antibody (M01), clone 2G1 (Cat # H00085366-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot detection against Immunogen (36.63 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MYLK2.
Immunogen
MYLK2 (NP_149109, 161 a.a. ~ 260 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
KLLAKKPPSEASELTFEGVPMTHSPTDPRPAKAEEGKNILAESQKEVGEKTPGQAGQAKMQGDTSRGIEFQAVPSEKSEVGQALCLTAREEDCFQILDDC
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (68)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.63 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MYLK2 expression in transfected 293T cell line by MYLK2 monoclonal antibody (M01), clone 2G1.
Lane 1: MYLK2 transfected lysate(64.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of MYLK2 over-expressed 293 cell line, cotransfected with MYLK2 Validated Chimera RNAi ( Cat # H00085366-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYLK2 monoclonal antibody (M01), clone 2G1 (Cat # H00085366-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — MYLK2
Entrez GeneID
85366GeneBank Accession#
NM_033118Protein Accession#
NP_149109Gene Name
MYLK2
Gene Alias
KMLC, MLCK, MLCK2, skMLCK
Gene Description
myosin light chain kinase 2
Gene Ontology
HyperlinkGene Summary
This gene encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle. [provided by RefSeq
Other Designations
OTTHUMP00000030560|OTTHUMP00000030561|OTTHUMP00000030562|myosin light chain kinase 2, skeletal muscle|skeletal muscle myosin light chain kinase|skeletal myosin light chain kinase
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Interactome
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Pathway
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Disease
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